One of the most essential functions of mitochondria is the generation of iron sulfur clusters essential within mitochondria and throughout the cell. Iron delivery to mitochondria can occur upon release of transferrin loaded iron from the early endosome compartments. In addition, excess cytosolic iron is stored by ferritin and must be recycled through ferritinophagy to regenerate the iron for delivery back to mitochondria. It has been shown that these transport events occur through regulated contact sites between endosomes and mitochondria, however very little is known about the mechanisms and regulation of these contacts. We have begun to explore these mechanisms and the impact of dynamic mitochondrial behavior and signaling on iron handling in multiple contexts.